abstract：:The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity...

journal_title：Journal of biotechnology

authors： Belghith H,Ellouz-Chaabouni S,Gargouri A

更新日期：2001-08-23 00:00:00

abstract：:A two-stage culture system was established for the production of astaxanthin from Haematococcus pluvialis. In a first stage green vegetative cells were produced in semicontinuous cultures maintained with daily renewal rates between 10 and 40%. The steady-state cell density decreased with increasing renewal rates. High...

journal_title：Journal of biotechnology

authors： Fábregas J,Otero A,Maseda A,Domínguez A

更新日期：2001-07-26 00:00:00

abstract：:beta-galactosidase reporter plasmids containing different viral or minimal promoters are commonly used to correct variable transfection efficiencies in transient transfection experiments. The transcriptional activity of these promoters is thought to be stable under most circumstances. To determine if expression of bet...

journal_title：Journal of biotechnology

authors： Huszár T,Mucsi I,Terebessy T,Masszi A,Adamkó S,Jeney C,Rosivall L

更新日期：2001-07-12 00:00:00

abstract：:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

journal_title：Journal of biotechnology

authors： Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

更新日期：2001-06-15 00:00:00

abstract：:Pimprinine, an extracellular alkaloid has been isolated from the culture filtrate of Streptomyces CDRIL-312. Pimprinine was subsequently purified using silica gel column chromatography and also by preparatory thin layer chromatography. Some physicochemical properties, antimicrobial activities (in-vitro) and pharmacolo...

journal_title：Journal of biotechnology

authors： Naik SR,Harindran J,Varde AB

更新日期：2001-06-01 00:00:00

abstract：:The efficiency of existing combinatorial biological library methods has been moderate in terms of the success rates, the affinities of the ligands selected and the time and effort involved in trying to optimize the initial leads. Although mimicking natural evolution, existing strategies take little notice of the impor...

journal_title：Journal of biotechnology

authors： Collins J,Horn N,Wadenbäck J,Szardenings M

更新日期：2001-06-01 00:00:00

abstract：:Endoglucanases (endo-1,4-beta-D-glucan-4-glucanohydrolase, EC 3.2.1.4) are industrially important enzymes. In this study endoglucanase I (EGI or Cel7B) of the filamentous fungi Trichoderma reesei has been genetically engineered to investigate the influence of tryptophan rich peptide extensions (tags) on partitioning i...

journal_title：Journal of biotechnology

authors： Collén A,Ward M,Tjerneld F,Stålbrand H

更新日期：2001-05-04 00:00:00

abstract：:Plant virus vectors provide an attractive biotechnological tool for the transient expression of foreign genes in whole plants. As yet there has been no use of recombinant viruses for the improvement of commercial crops. This is mainly because the viruses used to create vectors usually cause significant yield loss and ...

journal_title：Journal of biotechnology

authors： Arazi T,Slutsky SG,Shiboleth YM,Wang Y,Rubinstein M,Barak S,Yang J,Gal-On A

更新日期：2001-04-27 00:00:00

abstract：:A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules ...

journal_title：Journal of biotechnology

authors： Eggeling C,Berger S,Brand L,Fries JR,Schaffer J,Volkmer A,Seidel CA

更新日期：2001-04-13 00:00:00

abstract：:Often, degradability under anaerobic conditions is desirable for plastics claimed to be biodegradable, e.g. in anaerobic biowaste treatment plants, landfills and in natural anaerobic sediments. The biodegradation of the natural polyesters poly(beta-hydroxybutyrate) (PHB), poly(beta-hydroxybutyrate-co-11.6%-beta-hydrox...

journal_title：Journal of biotechnology

authors： Abou-Zeid DM,Müller RJ,Deckwer WD

更新日期：2001-03-30 00:00:00

abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

journal_title：Journal of biotechnology

authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

更新日期：2001-03-09 00:00:00

abstract：:This work explores the role of one of the factors explaining lipase/esterase activity: the contribution of electrostatic interactions to lipase/esterase activity. The electrostatic potential distribution on the molecular surface of an enzyme as a function of pH determines, to a large extent, the enzyme's pH activity p...

journal_title：Journal of biotechnology

authors： Neves Petersen MT,Fojan P,Petersen SB

更新日期：2001-02-13 00:00:00

abstract：:The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a mar...

journal_title：Journal of biotechnology

authors： Bensaddek L,Gillet F,Saucedo JE,Fliniaux MA

更新日期：2001-01-23 00:00:00

abstract：:Sulfite dissolving pulp from Eucalyptus grandis contained approximately 3.8% O-acetyl-4-O-methylglucuronoxylan with a molar ratio of xylose:4-O-methylglucuronic acid:acetyl group close to 13.6:1:6.2. The effects produced by purified endo-xylanases from two different glycosyl hydrolase families (family 10 and 11) as we...

journal_title：Journal of biotechnology

authors： Christov L,Biely P,Kalogeris E,Christakopoulos P,Prior BA,Bhat MK

更新日期：2000-10-13 00:00:00

abstract：:During the course of a lifetime, it has been calculated that we may consume between 100 and 700 tons of food. For the average British citizen, this is likely to include some 550 poultry, 36 pigs, 36 sheep, eight oxen, 10000 eggs and dairy products (milk, butter cheese, etc.) equivalent to 18 tonnes of milk. As if that...

journal_title：Journal of biotechnology

authors： Stokes CR,Bailey M

更新日期：2000-09-29 00:00:00

abstract：:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

journal_title：Journal of biotechnology

authors： Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

更新日期：2000-09-29 00:00:00

abstract：:A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at pos...

journal_title：Journal of biotechnology

authors： Yang Y,Jiang L,Zhu L,Wu Y,Yang S

更新日期：2000-08-25 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:Terminal sialic acid on oligosaccharides of glycoproteins shows several biological functions of the glycoproteins. The yeast Pichia pastoris normally does not contain sialic acid on the oligosaccharides of glycoproteins. A sialyltransferase (ST) gene was transfected into P. pastoris to assess the possibility of using ...

journal_title：Journal of biotechnology

authors： Chotigeat W,Chayanunnukul W,Phongdara A

更新日期：2000-07-28 00:00:00

abstract：:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

journal_title：Journal of biotechnology

authors： Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

更新日期：2000-07-14 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:Proteins are multifunctional: their amino acid sequences simultaneously determine folding, function and turnover. Correspondingly, evolution selected for compromises between rigidity (stability) and flexibility (folding/function/degradation), to the result that generally the free energy of stabilization of globular pr...

journal_title：Journal of biotechnology

authors： Jaenicke R

更新日期：2000-05-26 00:00:00

abstract：:One hundred and seventy-eight new butanol-acetone producing bacteria related to saccharolytic clostridia were isolated from agricultural sources in Colombia and their fermentation potential was evaluated. Thirteen isolates produced more total solvents from glucose than Clostridium acetobutylicum ATCC 824. The isolates...

journal_title：Journal of biotechnology

authors： Montoya D,Spitia S,Silva E,Schwarz WH

更新日期：2000-04-28 00:00:00

abstract：:The fed-batch process for commercial production of riboflavin (vitamin B2) was optimized on-line using model-predictive control based on artificial neural networks (ANNs). The information required for process models was extracted from both historical data and heuristic rules. After each cultivation the process model w...

journal_title：Journal of biotechnology

authors： Kovárová-Kovar K,Gehlen S,Kunze A,Keller T,Däniken RV,Kolb M,van Loon AP

更新日期：2000-04-14 00:00:00

abstract：:Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously del...

journal_title：Journal of biotechnology

authors： Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

更新日期：2000-03-31 00:00:00

abstract：:Flow cytometry was used to demonstrate the presence of beta-lactoglobulin (betaLG) receptors on living murine hybridoma MARK-3 cells using a fluorescein isothiocyanate-betaLG conjugate (FITC-betaLG: molar ratio of 5:1). A site occupation curve was produced using a shift in the mean channel fluorescence at various conc...

journal_title：Journal of biotechnology

authors： Palupi NS,Franck P,Guimont C,Linden G,Dumas D,Stoltz J,Nabet P,Belleville-Nabet F,Dousset B

更新日期：2000-03-10 00:00:00

abstract：:Combinatorial library selections through the systematic evolution of ligands by exponential enrichment (SELEX) technique identify so-called nucleic acid aptamers that bind with high-affinity and specificity to a wide range of selected molecules. However, the modest chemical functionality of nucleic acids poses some li...

journal_title：Journal of biotechnology

authors： Kusser W

更新日期：2000-03-01 00:00:00

abstract：:Proteolytically cleaved human 22 kDa growth hormone (22K hGH) between the amino acid residues 134 and 150 by plasmin or other proteases in vitro has been reported to be most active in growth promoting activity. In this study a deleted mutant hGH lacking amino acid residues from 135 to 146 and having more sensitivity t...

journal_title：Journal of biotechnology

authors： Alam KS,Fujikawa T,Yoshizato H,Tanaka M,Nakashima K

更新日期：2000-02-28 00:00:00

abstract：:A new, chemically defined medium providing dispersed growth and high biomass formation and a method for quantitative extraction of intracellular metabolites was used to investigate the cellular response of Streptomyces coelicolor A3(2) during growth and upon changes in nutrient utilization. Fast changes of the glucose...

journal_title：Journal of biotechnology

authors： Mira de Orduña R,Theobald U

更新日期：2000-02-17 00:00:00

abstract：:31P nuclear magnetic resonance (31P NMR) was used to monitor cytoplasmic and vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusion of the cell plug with...

journal_title：Journal of biotechnology

authors： Hesse SJ,Ruijter GJ,Dijkema C,Visser J

更新日期：2000-01-28 00:00:00

abstract：:A Pseudomonas aeruginosa biofilm is studied with pulsed field gradient echo nuclear magnetic resonance. Although not all spectral components are assigned yet, the experimental results show that a biofilm consists of components with very different diffusion coefficients. The various biofilm components that give motiona...

journal_title：Journal of biotechnology

authors： Vogt M,Flemming HC,Veeman WS

更新日期：2000-01-28 00:00:00

abstract：:In this paper we report the development of a recombinant strain of the yeast Pichia pastoris, which secretes an anti-carcinoembryonic antigen single chain Fv (scFv) antibody fragment to the culture supernatant as a biologically active protein, at levels of 1.2 g l(-1). The yeast scFv was purified by IMAC, with a final...

journal_title：Journal of biotechnology

authors： Freyre FM,Vázquez JE,Ayala M,Canaán-Haden L,Bell H,Rodríguez I,González A,Cintado A,Gavilondo JV

更新日期：2000-01-21 00:00:00

abstract：:Penicillium purpurogenum secretes arabinofuranosidase to the growth medium. Highest levels of enzyme (1.0 U ml(-1)) are obtained when L-arabitol is used as carbon source, while 0.85 and 0.7 U ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. By means of a zymogram, three bands with arabinofu...

journal_title：Journal of biotechnology

authors： De Ioannes P,Peirano A,Steiner J,Eyzaguirre J

更新日期：2000-01-21 00:00:00

abstract：:In monitoring and controlling wastewater treatment processes, on-line information of nutrient dynamics is very important. However, these variables are determined with a significant time delay. Although the final effluent quality can be analyzed after this delay, it is often too late to make proper adjustments. In this...

journal_title：Journal of biotechnology

authors： Lee DS,Park JM

更新日期：1999-10-08 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHi) is a gram-negative coccobacillus that is one of the bacteria that form the commensal flora of the upper respiratory tract in humans. This bacterium is an important human pathogen causing a broad spectrum of disease in both adults and children, including invasive and localised ...

journal_title：Journal of biotechnology

authors： Kyd J,Cripps A

更新日期：1999-08-20 00:00:00

abstract：:Feline immunodeficiency virus (FIV) infection is a naturally occurring lentiviral infection of cats which progresses to immunodeficiency in a manner strikingly similar to that observed in HIV infection in man. The rectal and cervico-vaginal mucosae are common routes of transmission of HIV and it has been shown that th...

journal_title：Journal of biotechnology

authors： Stokes CR,Finerty S,Gruffydd-Jones TJ,Sturgess CP,Harbour DA

更新日期：1999-08-20 00:00:00

abstract：:The energy which simple-stomached livestock can derive from dietary plant material is limited by the lack of plant polysaccharide degrading enzymes in their gastro-intestinal (GI) tract and the inefficient microbial fermentation of such material in their hind-gut. In poultry the non-starch polysaccharides found in cer...

journal_title：Journal of biotechnology

authors： Fontes CM,Ali S,Gilbert HJ,Hazlewood GP,Hirst BH,Hall J

更新日期：1999-06-11 00:00:00

abstract：:A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phe...

journal_title：Journal of biotechnology

authors： Ramírez DM,Bentley WE

更新日期：1999-05-28 00:00:00

abstract：:The quantitative description of the relationships between global properties, defined at the cellular population level, and individual properties, defined at the single cell level, is considered in this communication along with the analysis of some segregated models of yeast and hybridoma cell cultures. ...

journal_title：Journal of biotechnology

authors： Cazzador L

更新日期：1999-05-28 00:00:00

abstract：:In order to develop a serum-free (SF) medium for the production of erythropoietin (EPO) by suspension culture of recombinant Chinese hamster ovary (rCHO) cells, a statistical optimization approach based on a Plackett-Burman design was adopted. A basal medium was prepared by supplementing Iscove's modified Dulbecco's m...

journal_title：Journal of biotechnology

authors： Lee GM,Kim EJ,Kim NS,Yoon SK,Ahn YH,Song JY

更新日期：1999-04-15 00:00:00